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Oligonucleotide Melting Temperature
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SOLVED: A rudimentary method to calculate the melting temperature of DNA is an equation known as "The Wallace Rule." This equation is: Tm = 2(A + T) + 4(G + C) Where
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SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
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Le calcul avancé ; . TM = tangente = yjy, MNOT = ^- à l'origine de tangente = = x le calcul de ces résultats est suffisamment evi-dent de la figure. On peut noter que thesubtangent, normale, etc., sont des valuesfor un point donné de la courbe, mais peut être ...
SOLVED: The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal and depends directly on length and composition of the primer(s). An annealing temperature 5°C
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